The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. The tryptic peptides
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چکیده
منابع مشابه
The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptides from digestion with a staphylococcal proteinase.
The extracellular proteinase of Staphylococcus aureus strain V8 was used to digest the NADP-specific glutamate dehydrogenase of Neurospora crassa. Of 35 non-overlapping peptides expected from the glutamate content of the polypeptide chain, 29 were isolated and substantially sequenced. The sequences obtained were valuable in providing overlaps for the alignment of about two-thirds of the sequenc...
متن کاملAmino-acid sequence of NADP-specific glutamate dehydrogenase of neurospora crassa.
A tentative primary structure of the NADP-specific glutamate dehydrogenase [L-glutamate: NADP oxidoreductase (deaminating), EC 1.4.1.4] from Neurospora crassa has been determined. The proposed sequence contains 452 amino-acid residues in each of the identical subunits of the hexameric enzyme. Comparison of the sequence with that of the bovine liver enzyme reveals considerable homology in the am...
متن کاملPartial amino-acid sequence of NAD-specific glutamate dehydrogenase of Neurospora crassa.
Parts of the primary structure of the NAD-specific glutamate dehydrogenase [L-glutamate:NAD oxidoreductase (deaminating), EC 1.4.1.2] from Neurospora crassa are presented. Segments of the sequence representing 886 unique amino-acid residues have been determined; the largest contains 267 residues. There are only short regions of possible homology between this enzyme and the glutamate dehydrogena...
متن کاملThe amino acid sequence of the tryptic peptides isolated from dogfish M4 lactate dehydrogenase.
The peptides which result from treatment of the S-[14C]carboxymethyl derivative of dogfish M4 lactate dehydrogenase (EC 1.1.1.27) with trypsin have been isolated and their sequences have been elucidated. Each identical subunit has a molecular weight of 36,000 and on the basis of the amino acid composition 40 unique tryptic peptides are anticipated. Thirty-seven of these peptides have been isola...
متن کاملNeurospora crassa cytochrome c. II. Chymotryptic peptides, tryptic peptides, cyanogen bromide peptides, and the complete amino acid sequence.
The complete amino acid sequence of Neurospora crassa cytochrome c has been determined. The molecule consists of a single polypeptide chain containing 107 residues. The NH2-terminal residue is glycine, and the COOH-terminal residue is alanine. There are 39 amino acid differences between the cytochromes of N. crassa and those of the yeast Saccharomyces cerevisiae, whereas there are 43 amino acid...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0264-6021
DOI: 10.1042/bj1490739